Coding

Part:BBa_K1914000:Design

Designed by: Eloise Lloyd   Group: iGEM16_Exeter   (2016-10-07)


E. coli Codon Optimised KillerOrange(EOKO)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli K12 using IDT's online server


Source

Mutant of KillerRed, a red fluorescent protein from jellyfish Anthomedusae sp chromoprotein anm2CP, produced by random mutatagenesis using an error-prone PCR. Sarkisyan, K.S., Zlobovskaya, O.A., Gorbachev, D.A., Bozhanova, N.G., Sharonov, G.V., Staroverov, D.B., Egorov, E.S., Ryabova, A.V., Solntsev, K.M., Mishin, A.S. and Lukyanov, K.A., 2015. KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. PloS one,10(12), p.e0145287.

References